Composite

Part:BBa_K2022008:Design

Designed by: Gavin Sutton   Group: iGEM16_UNSW_Australia   (2016-10-13)


T7 Expression Construct for tolR, Periplasmic Domain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We opted to use a T7 promoter to express g3p, as in many expression strains of E. coli (those that are DE3), T7 polymerase is IPTG-inducible. This, ultimately, allows the control of tolR expression, which is critical when the protein has the potential to harm the cell if overexpressed to too great an extent (as one could imagine being the case with vesicle formation)


Source

An assembly of BBa_K525998 - BBa_K2022002 - BBa_B0017

References